Device

Part:BBa_K185018

Designed by: Shikun Zhao   Group: iGEM09_SJTU-BioX-Shanghai   (2009-10-16)

Promoter118+QPI+RBS30+RelB+Double Terminator

This part is based on QPI(Q04510), which made by early team. We add a promoter(J23118) on the begining of QPI and add RBS(B0030), RelB(K185048) gene and Double-terminator(B0015) at the end of it in order to express RelB protein. Because of QPI contain CI gene so that this device can be control by CI protein.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1162
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None